Publications
In order to view the full text of the publications
(in pdf format), Adobe Acrobat Reader is required. Follow the link at the bottom
of the page.
Enzymatic tunneling and kinetic isotope effects: chemistry at the crossroads, Sen, A., and Kohen, A.*, J. Phys. Org. Chem. (invited review for a special issue entitled “Symposium in Print on Tunneling”; Accepted for publication Sept. 16, 2009).
Effect of electrostatic shielding on H-tunneling in R67 dihydrofolate, Yahashiri, A.; Nimrod, G.; Ben-Tal, N.; Howell, E.E.; and Kohen, A.*, ChemBioChem 2009, 10, 2620-2623
Flavin-dependent thymidylate synthase: A novel pathway towards thymine, Koehn, E.M. and Kohen, A.*, Arch Biochem Biophys.(Available online Aug. 15, 2009).
Exploring the Molecular Origins of Protein Dynamics in the Active Site of Human Carbonic Anhydrase II, Hill, S.E., Bandaria, J.N., Fox, M., Vanderah, E., Kohen A.,and Cheatum, C.M.*, J. Phys. Chem. B. 2009, 113, 11505 - 11510.
Microscale synthesis and kinetic isotope effect analysis of (4R)-[Ad-14C, 4-2H] NADPH and (4R)-[Ad-3H,4-2H] NADPH, Yahashiri, A.; Sen, A.; and Kohen, A.*, J. Labeled Comp. Radiopharma, 2009, 52, 463-466.
Examination of Enzymatic H-Tunneling through Kinetics and Dynamics, Bandaria, J.N., Cheatum, C.M., and Kohen, A.*, J. Am. Chem. Soc. 2009, 131, 10151-10155
An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene, Koehn, E.M., Fleischmann, T., Conrad, J.A., Palfey, B.A., Lesley, S.A., Mathews, I.I., and Kohen, A.*, Nature 2009, 458, 919-923.
Oxidase Activity of a Flavin-Dependent Thymidylate Synthase, Wang, Z., Chernyshev, A., Koehn, E.M., Manuel, A., Lesley, S.A., and Kohen A.*, FEBS J. 2009, 276 (10), 2801-2810.
Enzymatic H-transfers: Quantum Tunneling and Coupled Motion from Kinetic Isotope Effect Studies, Stojkovic, V.;Kohen A.*, Isr. J. Chem. 2009, 49, 163-173 (Invited Review).
Quantum Effects in Enzyme Kinetics, Sen, A., and Kohen, A.*, in Quantum Tunnelling in Enzyme Catalyzed Reactions, Allemann, R. and Scrutton, N., Eds, Royal Society of Chemistry, London, UK, 2009, Ch. 7, pp.164-181. ISBN 978-0-85404-122-0
QM/MM study of Thymidylate Synthase: Enzymatic motions and the temperature dependence of the rate limiting step, Kanaan, N., Marti, S.*, Moliner, V.*, and Kohen, A., J. Phys. Chem. A. 2009, 113, 2176-2182 (Special Issue on Chemical Dynamics: Max Wolfsberg Festschrift, Sharon Hammes-Schiffer Ed.).
Kohen A., Spotlight: ³Deep Tunneling Dominates the Biologically Important Hydride Transfer Reaction from NADH to FMN in Morphinone Reductase² J. Am. Chem. Soc. 2008, (invited Spotlight on JACS 2008, 130, 7092).
Tuning of the H-Transfer Coordinate in Primitive versus Well-Evolved Enzymes, Yahashiri, A., Howell, E.E., and Kohen, A.*, ChemPhysChem 2008, 9, 980-982 (Communication). Supplementary Information.
Fast Enzyme Dynamics at the Active Site of Formate Dehydrogenase, Bandaria, J., Dutta, S., Hill, S., Kohen, A., and Cheatum, C.*, J. Am. Chem. Soc. 2008, 130, 22-23 (Communication).
The Role of Y94 in Proton and Hydride Transfers Catalyzed by Thymidylate Synthase Hong, B., Maley, F., and Kohen, A.*, Biochemistry 2007, 46, 14188-14197.
The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS) Chernyshev, A., Fleischmann,T., Koehn, E.M., Lesley, S.A., and Kohen, A.*, Chem. Commun., 2007, 2861-2863.
A QM/MM Study of the catalytic mechanism of the thymidylate synthase, Kanaan, N., Mart’, S.*, Moliner, V.*, and Kohen, A., Biochemistry, 2007, 46, 3704-3713.
Thymidyl Biosynthesis Enzymes as Antibiotic Targets, Chernyshev, A., Fleischmann, T., and Kohen, A.*, Appl. Microbiol. Biotech., 2007, 74. 282-289 (review).
Probes for hydrogen tunneling and coupled motion in enzymatic systems, Kohen, A. in Hydrogen-Transfer Reactions, Vol; 4: Biological Aspect , Hynes, Klinman, Limbach, Schowen, Eds., Wiley_VCH, Weinheim, 2007, Chapter 12, pp. 1311-1340.
Coordinated Effects of Distal Mutations on Environmentally Coupled Tunneling in Dihydrofolate Reductase, Wang, L., Goodey, N.M., Benkovic, S.J.*, and Kohen, A.*, Proc. Natl. Acad. Sci. USA 2006, 103, 15753-15758.
Analytical Procedures for the Preparation, Isolation, Analysis and Preservation of Reduced Nicotinamides Markham, K.A., and Kohen, A.*, Curr. Anal. Chem., 2006, 2, 379-388 (invited review).
Kinetic isotope effects as probes for hydrogen tunneling in enzyme catalysis Kohen, A., in Isotope Effects in Chemistry and Biology, Kohen, A. Limbach, H.H. Eds., Taylor & Francis -CRC Press, Boca Raton, FL, 2006, Ch. 28, pp-743-764. ISBN 0824724496.
Hydride transfer versus hydrogen radical transfer in thymidylate synthase Hong, B., Haddad, M., Maley, F., Jensen, J.H., and Kohen, A.* J. Am. Chem. Soc. (communication) 2006, 128, 5636 - 5637 Supplementary Information
A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link Mason, A., Agrawal, N., Washington, T., Lesley, S.A., and Kohen, A.*, Chem. Comm. 2006, 1781Ð1783.
Arrhenius curves of hydrogen transfers: Tunnel effects, isotope effects, and effects of pre-equilibria Limbach, H.H., Lopez, J.M., and Kohen, A., Phyl. Trans. R. Soc. B., 2006, 361,1399 - 1415.
The role of enzyme dynamics and tunneling in catalyzing hydride transfer: Studies of distal mutants of dihydrofolate reductase Wang, L., Goodey, N.M., Benkovic, S.J., and Kohen, A., Phyl. Trans. R. Soc. B., 2006, 361, 1307-1215. Supplementary Information
Effects of a distal mutation on active site chemistry Wang, L., Tharp, S., Selzer, T., Benkovic, S.J., and Kohen, A., Biochemistry, 2006, 45, 1383-1392. Supplementary Information
Nonperfect synchronization of reaction center rehybridization in the transition state of the hydride transfer catalyzed by dihydrofolate reductase Pu, J., Ma, S., Garcia-Viloca, M., Gao, J., Truhlar, D.G., and Kohen, A., J. Am. Chem. Soc., 2005, 127, 14879-14886.
Microscale synthesis of isotopically labeled 6R-N5, N10 methylene-5, 6, 7, 8-tetrahydrofolate Hong, B. and Kohen, A., J. Label Compd. Radiopharm. 2005, 48, 759Ð769.
Competitive 15N kinetic isotope effects of nitrogenase catalyzed dinitrogen reduction Sra, A.K., Hu, Y. Martin, G.E., Snow, D.D., Ribbe, M.W., and Kohen, A. J. Am. Chem. Soc. 2004, 126, 12768-12769; Supplementary Information
Mechanistic studies of a flavin-dependent thymidylate synthase Agrawal, N., Lesley, S.A., Kuhn, P., Kohen, A. Biochemistry, 2004, 43, 10295-10301, Accelarated Publication; Supplementary Information.
Tunneling and coupled motion in the E. coli dihydrofolate reductase catalysis R. Steven Sikorski, Lin Wang, Kelli A. Markham, P. T. Ravi Rajagopalan, Stephen J. Benkovic, and Amnon Kohen J. Am. Chem. Soc. 2004, 126, 4778-4779; Supplementary Information
Microscale synthesis of isotopically labeled R-[6-XH]-N5,N10-methlene 5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase Agrawal, N., Mihai, C., Kohen, A. Anal. Biochem.2004 328, 44-50.
Vibrationally enhanced hydrogen tunneling in the E. coli thymidylate synthase catalyzed reaction Agrawal, N., Hong, B., Mihai, C., and Kohen, A. Biochemistry, 2004, 43, 1998-2006.
Synthesis and utility of 14C-nicotinamide cofactors Markham, K., Sikorski, R.S., and Kohen, A. Anal. Biochem. 2004, 325, 62-67
Synthesis of R and S tritiated reduced b-nicotinamide adenine dinucleotide 2' phosphate McCracken, J. A., Wang, L., Kohen, A. Anal. Biochem 2003, 324, 131-136
Microscale synthesis of 2-tritiated isopropanol and 4-R tritiated reduced nicotinamide adenine dinucleotide phosphate Agrawal, N. and Kohen, A. Anal. Biochem. 2003, 322, 179-184
Purification, analysis and preservation of reduced nicotinamide adenine dinucleotide 2'-phosphate Markham, K.A., Sikorski, R.S., and Kohen, A. Anal. Biochem. 2003, 322, 26-32
Kinetic isotope effects as probes for hydrogen tunneling, coupled motion and dynamics contributions to enzyme catalysis, A. Kohen Prog. React. Kinet. Mech. 2003 28, 119-156
Nitrogenase catalyzed dinitrogen reduction -a new mechanistic approach, Sra, A.K.; Kohen, A., Nitrogen Fixation, Global Perspective, 2002, (eds. T Finan, M O'Brian, D Layzell, K Vessey, W Newton), CABI Publishing, London, p 371.
Boundary Conditions for the Swain-Schaad Relationship as a Criterion for Hydrogen Tunneling, Kohen, A.; Jensen, J. J. Am. Chem. Soc., 2002,124(15); 3858-3864.
Convex Arrhenius plots and their interpretation, Truhlar, D. G., and Kohen, A. Proc. Natl. Acad. Sci. USA, 2001, 98, 848-851.
Protein flexibility correlates with degree of hydrogen tunneling in thermophilic and mesophilic alcohol dehydrogenases, Kohen, A.; J. P. Klinman J. Am. Chem. Soc., 2000, 122, 10738-10739.
Hydrogen Tunneling in Biology, Kohen, A.; Klinman, J. P. Chemistry and Biology 1999, 6, R191-198. [review]
Enzyme Dynamics and Hydrogen Tunneling in a Thermophilic Alcohol Dehydrogenase, Kohen, A.; Cannio, R.; Bartolucci, S.; Klinman, J. P. Nature 1999, 399, 496-499.
Enzyme Catalysis: Beyond Classical Paradigms, Kohen, A.; Klinman, P. J. Acc. Chem. Res. 1998, 31, 397-404. [review]
Catalytic Mechanism of KDO8P Synthase: Transient Kinetic Studies and Evaluation of a Putative Reaction Intermediate., Liang, P. H.; Lewis, J.; Anderson, K. S.; Kohen, A.; D'Souza, F. W.; Benenson, Y.; Baasov, T. Biochemistry 1998, 37, 16390-16399.
Effect of Protein Glycosylation on Catalysis: Changes in Hydrogen Tunneling and Enthalpy of Activation in the Glucose Oxidase Reaction., Kohen, A.; Jonsson, T.; Klinman, P. J. Biochemistry 1997, 36, 2603-2611.
Effect of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation, Kohen, A.; Jonsson, T.; Klinman, J. P. Tech. Prot. Chem 1997, VIII, 311-319.
Catalytic Mechanism of KDO8P Synthase. Pre-Steady-State Kinetic Analysis Using Rapid Chemical Quench Flow Methods, Liang, P. H.; Kohen, A.; Baasov, T.; Anderson, K. S. Bioorganic & Medical Chem. Lett. 1997, 7, 2463-2468.
Synthesis, Inhibition and Acid-Catalyzed Hydrolysis Studies of Model Compounds of the Proposed Intermediate in the KDO8P Synthase Catalyzed Reaction, Baasov, T.; Kohen, A. J. Am. Chem. Soc. 1995, 117, 6165-6174.